Research Technician – Microbiology The Center for Wildlife Health located in the Institute of Agriculture at the University of Tennessee-Knoxville (UTK) is seeking a part-time Research Technician to assist with the diagnostics of wildlife pathogens. This is a grant-funded research position for a one-year term, with continued renewal dependent upon the availability of future funding. The individual will work with a multi-disciplinary and multi-institutional team investigating the pathogenicity and transmission dynamics of the recently discovered chytrid fungus, Batrachochytrium salamandrivorans (Bsal). The individual will perform DNA extractions, quantitative PCR, and prepare H&E slides from amphibian samples collected during controlled laboratory experiments. Culturing Bsal for experiments also will be a responsibility. Immediate supervisors will be Drs. Matthew Gray, Patrick Cusaac, and Debra Miller. This is a temporary (<30-hours per week), hourly position with no benefits. UTK pay grade 32-34, dependent upon selected candidate's qualifications. Review of applications will begin September 1, 2017, and remain open until filled.
Qualifications Education: High school (HS) diploma or equivalent; post-HS degree preferred. Experience: Molecular and microbiological techniques used in culturing and detecting pathogens and diagnosing diseases. The successful candidate will be required to extract genomic DNA from animal tissues and swabs using a Qiagen DNeasy kit, quantify DNA using a Qubit fluorometer and Quant-iT dsDNA BR Assay Kit, perform quantitative polymerase chain reaction (qPCR) using a ABI Prism 7900HT, and analyze qPCR results using Quantstudio software. The candidate also will prepare Hematoxylin and Eosin (H&E) stained slides for light microscopy and assist with culturing Bsal in agar plates. Preferred Job Skills: Molecular and microbiological skills, sterile laboratory procedures, organizational and data recording skills, and experience with qPCR, cell culture, histology, and flow cytometry.
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